丰满中国
发表于 2025-3-28 16:19:06
RNA Visualization in Bacteria by Fluorescence In Situ Hybridization microscopy. This chapter describes the use of FISH to visualize tmRNA, a regulatory RNA required for .-translation. The method can be adapted to visualize the localization of other regulatory and messenger RNAs as well.
郊外
发表于 2025-3-28 21:09:54
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Parallel
发表于 2025-3-29 00:25:55
Gel Mobility Shift Assays to Detect Protein–RNA Interactionsative polyacrylamide gels in Tris/borate/EDTA buffer, although an alternative Tris-glycine buffering system is superior in many situations. Here, we describe both gel shift methods, along with strategies to improve separation of protein–RNA complexes from free RNA, which can be a particular challenge for small RNA binding proteins.
阻塞
发表于 2025-3-29 04:23:34
RNase Footprinting of Protein Binding Sites on an mRNA Target of Small RNAsucleotides, to footprint the protein Hfq on the . mRNA leader. This protocol covers how to form the RNP complex, determine the correct dose of enzyme, footprint the protein, and analyze the cleavage pattern using primer extension.
盲信者
发表于 2025-3-29 11:15:01
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Gene408
发表于 2025-3-29 13:15:04
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执
发表于 2025-3-29 15:49:38
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exclusice
发表于 2025-3-29 23:10:01
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BARGE
发表于 2025-3-30 01:33:26
Use of Semi-quantitative Northern Blot Analysis to Determine Relative Quantities of Bacterial CRISPR stabilities of both pre-crRNA and crRNA. The procedures described in this chapter can be used with very minor modifications to monitor the abundance and stabilities of transcripts of various lengths from many bacterial sources.
Scleroderma
发表于 2025-3-30 06:48:45
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