fibroblast
发表于 2025-3-25 03:54:00
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碎石
发表于 2025-3-25 10:28:25
Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter Acrm in cellular level. These pumps not only confer multidrug resistance of cancer cells and pathogenic bacteria but also cause hereditary diseases through the mutation. Our purposes are to elucidate the molecular structures and mechanisms of these xenobiotic exporters..We had succeeded to determine th
rheumatology
发表于 2025-3-25 14:46:11
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Basilar-Artery
发表于 2025-3-25 16:54:32
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缺陷
发表于 2025-3-25 22:52:30
Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysisdrug transporter AcrAB-TolC, which uses proton electrochemical gradients to vectorially drive the efflux of drugs from the cell, spans this envelope. We describe here details of the methods used to prepare the recombinant tripartite assembly for high-resolution structure determination by cryo-EM.
goodwill
发表于 2025-3-26 02:33:57
NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exportance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.
gnarled
发表于 2025-3-26 06:00:19
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maintenance
发表于 2025-3-26 08:45:18
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过分自信
发表于 2025-3-26 16:29:34
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organism
发表于 2025-3-26 18:59:11
Determining Ligand Path Through a Major Drug Transporter, AcrB, in ,theoretical considerations. The protocols for bacterial culture preparation, membrane protein purification, fluorescent assay standardization, data collection, and data analysis are provided. Useful software tools are recommended.