咆哮
发表于 2025-3-23 12:48:50
All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteinse provide general protocols for mutagenesis of a target codon in a plasmid-borne gene and for the selection and screening of the resultant mutants. These techniques should be adaptable for the study of a variety of bacterial proteins.
Brain-Waves
发表于 2025-3-23 15:01:12
Book 2018odel species, such as .Escherichia coli. and .Salmonella typhimurium., while being also applicable to a wide spectrum of other species. Beginning with an introduction, the sections of the book explore methods for studying bacterial chemotaxis at the population and whole-cell levels, in vivo analysis
枕垫
发表于 2025-3-23 20:57:44
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袖章
发表于 2025-3-24 01:54:48
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Indict
发表于 2025-3-24 04:19:03
Kseniya Katsman,Elizabeth L. Jeglicre captured on the membrane and imaged using confocal laser scanning microscopy (CLSM). Chemotaxis toward specific molecules produced by the biofilm, such as autoinducer-2 (AI-2), can be studied using this setup. This system can be adapted to study chemotaxis toward poly-species biofilms, or even mammalian cells.
jocular
发表于 2025-3-24 06:44:18
https://doi.org/10.1007/978-1-4615-1289-9 If filaments are fluorescent, they can be imaged in their entirety using standard fluorescence microscopes. For imaging in vivo, blurring can be prevented by strobing the light source or by using a camera with a fast shutter. The former method is preferred, since it minimizes bleaching.
渐强
发表于 2025-3-24 10:57:09
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harmony
发表于 2025-3-24 17:50:43
Visualizing Chemoattraction of Planktonic Cells to a Biofilmre captured on the membrane and imaged using confocal laser scanning microscopy (CLSM). Chemotaxis toward specific molecules produced by the biofilm, such as autoinducer-2 (AI-2), can be studied using this setup. This system can be adapted to study chemotaxis toward poly-species biofilms, or even mammalian cells.
Hamper
发表于 2025-3-24 20:21:07
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stress-response
发表于 2025-3-25 00:03:48
Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readouta FRET-based assay that uses signaling components of the . chemotaxis system. We demonstrate the approach with NarX, a nitrate/nitrite sensor kinase, but the method should be applicable to other two-component sensor kinases.