国家明智
发表于 2025-3-23 11:43:04
https://doi.org/10.1007/978-3-658-16013-5autophagy and its effects on cell function. We also discuss potential pitfalls. Although cell lines are used as model systems, the substances and methods described here can be applied to primary cells and tissues.
Opponent
发表于 2025-3-23 14:26:44
Gravity Field and Dynamics of the Earthquantified in a presentation assay with antigen-specific CD4. T-cell clones. These assays are good measures of autophagosome formation and lysosomal degradation of macroautophagy cargo and therefore are useful for studying regulation of the autophagic pathway under various experimental conditions and physiological perturbations.
Fibroid
发表于 2025-3-23 19:55:30
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REIGN
发表于 2025-3-24 01:34:06
Wolfgang Jacoby Johannes,Peter L. Smilde loaded endosomes and magnetic streptavidin conjugated bead-containing phagosomes. Fusion is quantified using a fluorescence-based detection method that measures the peroxidase activity associated with the beads.
翅膀拍动
发表于 2025-3-24 03:15:25
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卜闻
发表于 2025-3-24 07:37:47
LC3 and Autophagy, starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death. Here we describe basic protocols to assay for endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.
disparage
发表于 2025-3-24 13:44:44
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征税
发表于 2025-3-24 18:20:03
Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy,quantified in a presentation assay with antigen-specific CD4. T-cell clones. These assays are good measures of autophagosome formation and lysosomal degradation of macroautophagy cargo and therefore are useful for studying regulation of the autophagic pathway under various experimental conditions and physiological perturbations.
平淡而无味
发表于 2025-3-24 19:47:02
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使乳化
发表于 2025-3-24 23:44:15
,In Vitro Phagosome–Endosome Fusion, loaded endosomes and magnetic streptavidin conjugated bead-containing phagosomes. Fusion is quantified using a fluorescence-based detection method that measures the peroxidase activity associated with the beads.