vanquish 发表于 2025-3-25 05:21:27
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Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells is applicable both to . and the tobacco BY2 cell lines. Cell-cycle synchronization capacity of the parental lines is maintained after both transformation and recovery from cryopreservation. The techniques described here require no specialized equipment and are suitable for routine laboratory use, g关心 发表于 2025-3-25 14:28:58
Gene Identification and Cloning by Molecular Marker Mappingne fine-scale genetic linkage, ideally narrowing to a chromosomal region of about 40 kbp; and (4) sequencing of mutant and wild-type DNA is used to verify the identity of the mutated gene. Given a mutant phenotype that can be determined unambiguously in a single F2 plant, it is possible to completeManifest 发表于 2025-3-25 17:54:31
PCR-Based Screening for Insertional Mutantsonce again, are becoming valuable for obtaining loss-of-function alleles in the genes “missed” by the sequencing projects. In this chapter, we provide a detailed description of the design and implementation of a PCR-based screen for insertional knockouts using T-DNA-mutagenized lines as an example.magenta 发表于 2025-3-25 21:25:23
1064-3745 . Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the 978-1-61737-539-2978-1-59745-003-4Series ISSN 1064-3745 Series E-ISSN 1940-6029喃喃诉苦 发表于 2025-3-26 00:35:58
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Empirische Forschung und Waldorfpädagogikescribe standard cytogenetic methods including preparation of chromosomes and chromatin fibers, isolation of interphase nuclei, FISH and immunostaining, painting of . chromosomes, and comparative chromosome painting in other . species.