MULTI
发表于 2025-3-25 05:06:54
Future Directions,pments in mass spectrometry instrumentation. Two notable problems at this time are the broader acceptance of selected reaction monitoring as a primary method of protein quantitation and at least some improvement in the sensitivity of the analysis. Improvements will always include the continuing evol
破布
发表于 2025-3-25 09:31:05
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BLANC
发表于 2025-3-25 12:14:09
Michael Kinter,Caroline S. KinterExplains both the theoretical and experimental details of the selected reaction monitoring experiment as it is applied to proteins?.Describes a new type of quantitative proteomics experiment called se
中和
发表于 2025-3-25 19:26:47
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condescend
发表于 2025-3-25 23:14:01
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics978-1-4614-8666-4Series ISSN 2193-4746 Series E-ISSN 2193-4754
Bucket
发表于 2025-3-26 03:54:44
J. G. Brown,R. D. (Shawn) Blantontaken to find an optimal number of targets so that proper sampling of the chromatographic experiment is still obtained. The final challenge is then analyzing the data to determine the signals for the respective peptides and calculating an amount of each protein. Internal standards and housekeeping proteins facilitate this process.
两种语言
发表于 2025-3-26 06:51:53
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conspicuous
发表于 2025-3-26 09:55:44
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绕着哥哥问
发表于 2025-3-26 14:00:56
https://doi.org/10.1007/978-0-387-74747-7 act as the quantitative surrogate for each protein being measured. The overarching goal of the design process is find the best peptides to monitor and optimize the collision induced dissociation conditions to give the best signal for those peptides. The general stages are finding an initial set of
少量
发表于 2025-3-26 20:05:53
F. Su,W. Hwang,A. Mukherjee,K. Chakrabarty largely purified peptides injected into the LC-tandem mass spectrometry system. As seen with essentially any advanced analytical technique, the overall success of the selected reaction monitoring experiment is dependent on the quality of these samples. The accuracy and precision of the assay requir