Panacea
发表于 2025-3-23 12:13:57
Book 2019Latest editionnic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approac
Functional
发表于 2025-3-23 16:48:57
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致敬
发表于 2025-3-23 19:10:01
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责难
发表于 2025-3-24 00:19:01
https://doi.org/10.1007/978-3-662-33043-2eral diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.
不可接触
发表于 2025-3-24 03:01:29
Chapter 3 The Togetherness of Trustce proteins can be used to precisely probe various aspects of antigen presentation, including the kinetics of peptide generation, MHC class I surface stability, and presentation efficiency following pharmacological and genetic manipulations including genome wide and high throughput drug screening.
Introvert
发表于 2025-3-24 07:51:29
Shiu Yeh Yu,C. N. Sun,Murtie F. Stillreview the existing strategies for the isolation of MHC-restricted peptides and provide a detailed protocol for the immunoaffinity purification of MHC class I- and II-presented peptides from primary tissues or cells.
STELL
发表于 2025-3-24 14:19:54
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刀锋
发表于 2025-3-24 15:07:33
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捕鲸鱼叉
发表于 2025-3-24 19:22:31
Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes,g. Here, we describe the purification of active human 20S and 26S proteasomes from human erythrocytes by DEAE-ion exchange chromatography, ammonium sulfate precipitation, glycerol density gradient centrifugation, and Superose-6 size exclusion chromatography and their characterization using fluorogenic substrates and specific inhibitors.
在驾驶
发表于 2025-3-24 23:56:04
Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases,eral diseases including autoimmune diseases, viral infections, and virally induced cancers. In this chapter, we describe two basic methods for monitoring peptide-trimming activity by ER aminopeptidases and screening potential chemical inhibitors.