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Book 2018Latest editiondily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Antibody Engineering: Methods and Protocols, Third Edition. presents the reader with an extensive toolbox to create the powerful molecules of tomorrow..暴露他抗议 发表于 2025-3-30 19:00:39
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Antibody Affinity Maturation by Computational Designgen encounter by somatically mutating their genome sequences. The end result is a series of antibodies that display higher affinities and specificities to specific antigens. This process is called affinity maturation. Recent improvements in computer hardware and modeling algorithms now enable the ra不容置疑 发表于 2025-3-31 10:02:22
Use of IMGT® Databases and Tools for Antibody Engineering and Humanizationnage the huge diversity of the antigen receptors, immunoglobulins (IG) or antibodies, and T cell receptors (TR). The founding of IMGT. marked the advent of immunoinformatics, which emerged at the interface between immunogenetics and bioinformatics. Standardized sequence and structure analysis of ant彻底明白 发表于 2025-3-31 13:27:19
Construction of Human Naïve Antibody Gene Librariesens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human naïve antibody gene library for the selection of antibodies as single-chain variable fragments.树木中 发表于 2025-3-31 19:41:11
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Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation n binding in case of IgNAR antibodies is mediated by a single variable domain (vNAR). Due to their inherent beneficial biophysical properties, such as small size and high thermal stability combined with a high specificity and affinity to their target antigens, vNAR domains emerged as promising tools