FIS
发表于 2025-4-1 05:55:41
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condescend
发表于 2025-4-1 06:00:37
https://doi.org/10.1007/978-3-7091-7730-3addition of isotopically labeled standard amino acids and analysis by mass spectrometry for accurate and sensitive protein quantitation. Quantitation of less than 10 fmol of protein standards with errors below 10% has been demonstrated using this method.
Mangle
发表于 2025-4-1 13:19:06
https://doi.org/10.1007/978-3-7091-7730-3 be purified and their quantity and/or concentration precisely determined. In this chapter, we describe a protocol for the quantification of purified recombinant proteins. The protocol is based on a microwave-assisted acidic hydrolysis of the target protein followed by high-resolution mass spectrome