否认
发表于 2025-3-28 18:29:07
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时代错误
发表于 2025-3-28 20:57:36
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军火
发表于 2025-3-29 00:04:19
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Cultivate
发表于 2025-3-29 04:44:06
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Vulnerable
发表于 2025-3-29 07:49:44
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滴注
发表于 2025-3-29 11:52:19
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雇佣兵
发表于 2025-3-29 15:43:53
Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectr tools to specifically detect and quantify proteoforms (Smith et al., Nat Methods 10:186–187, 2013) is a major impediment to functional studies. Recently, biological mass spectrometry (MS) has undergone impressive advances (Mann, Nat Rev Mol Cell Biol 17:678, 2016), including the generation of a hig
可憎
发表于 2025-3-29 21:11:40
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engrossed
发表于 2025-3-30 02:29:56
Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention,a wide variety of genes controlled by IR, few techniques are available to identify regulators of IR in an unbiased manner. Here, we describe a CRISPR knockout screening method that can be applied to uncover regulators of IR. This method uses GFP reporter constructs containing a retained intron from
走调
发表于 2025-3-30 04:18:59
Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins, To elucidate the effect of a specific RBP on bound RNA, it can be artificially recruited to a specific site on a reporter mRNA that can be followed by a variety of methods. In this so-called tethering assay, the protein of interest (POI) is fused to the coat protein of the MS2 bacteriophage and exp