描绘 发表于 2025-3-25 06:39:31
https://doi.org/10.1007/978-3-030-59169-4in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in . has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phen冲突 发表于 2025-3-25 11:09:45
http://reply.papertrans.cn/16/1540/153908/153908_22.pngneurologist 发表于 2025-3-25 13:42:14
R. Hamid,Y. Khairullah,A. R. Khalimin the immediate vicinity of αSyn in living rat cortical neurons. To isolate these interactions, we transduced primary cortical neurons with a lentivirus encoding APEX2 tagged to the C-terminus of alpha-synuclein (αSyn) and under the control of a synapsin promoter. Neural protein lysates were then i捏造 发表于 2025-3-25 18:23:44
A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.擦试不掉 发表于 2025-3-25 21:07:48
http://reply.papertrans.cn/16/1540/153908/153908_25.png我不重要 发表于 2025-3-26 03:50:39
Methods in Molecular Biologyhttp://image.papertrans.cn/a/image/153908.jpg壮观的游行 发表于 2025-3-26 05:22:09
http://reply.papertrans.cn/16/1540/153908/153908_27.png我正派 发表于 2025-3-26 08:33:05
Developments in Injection Moulding—3 . in diseased brain has been a challenge in the field for some time. Here we describe a method, the alpha-synuclein proximity ligation assay (AS-PLA), to detect alpha-synuclein oligomers in paraffin-embedded brain sections. Using AS-PLA previously unobserved alpha-synuclein oligomeric pathology is revealed.油毡 发表于 2025-3-26 15:53:14
http://reply.papertrans.cn/16/1540/153908/153908_29.pngInculcate 发表于 2025-3-26 19:38:10
A. I. Yilmaz,U. Albostan,A. Caner,O. Kurc interactions of intrinsically disordered proteins as alpha-synuclein..Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis.