Cabinet
发表于 2025-3-27 00:52:02
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喃喃诉苦
发表于 2025-3-27 04:49:42
Rationally Designed Ligands for Use in Affinity Chromatography of antibodies. The concepts of rational design and solid-phase combinatorial chemistry were used for the discovery of a synthetic PpL mimic affinity ligand. The procedure presented in this chapter represents a general approach with the potential to be applied to different systems and target proteins.
BLANC
发表于 2025-3-27 09:09:55
Preparation, Analysis and Use of an Affinity Adsorbent for the Purification of GST Fusion Protein protein displays a dissociation constant of 0.6 x10. M to glutathione immobilized to Streamline™. Ligand density optimization, fusion protein elution conditions (pH and glutathione concentration) and ligand orientation are briefly discussed.
责任
发表于 2025-3-27 11:38:46
Corporate Venturing in der Praxisthentic epitope mimic and coupling the peptide mimotope to an affinity resin for purifying antibodies from human serum. There are several other applications of phage peptides that could be used for affinity chromatography; the approaches are outlined, but detailed methods have not been included.
丑恶
发表于 2025-3-27 16:51:11
Das Unternehmen im Stakeholder-Dialoghich ligand is bound to it. Here, we describe a procedure for immobilization of HaloTag™ fusion proteins and how immobilized proteins can be used to study protein–protein and protein–DNA interactions in vivo and in vitro.
Forsake
发表于 2025-3-27 20:40:01
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玩忽职守
发表于 2025-3-27 22:10:25
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags The UZ-HT15 tag and the method can be applied to virtually any protein. A description of the cloning strategy for the design of the genetic construction, two alternative approaches and a simple test to assess the performance of the tag removal process are also included.
繁荣地区
发表于 2025-3-28 03:52:07
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侵略
发表于 2025-3-28 09:41:48
Immobilized Metal Ion Affinity Chromatography of Native Proteinsm, that of the interaction between histidine and immobilized metal ions, those that enable purification of proteins that lack surface histidines and the operation of IMAC supports in cation exchange mode.
开花期女
发表于 2025-3-28 13:28:52
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