NOTCH
发表于 2025-3-23 13:31:29
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falsehood
发表于 2025-3-23 16:09:31
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渐变
发表于 2025-3-23 19:42:05
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ETHER
发表于 2025-3-24 02:08:01
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厨师
发表于 2025-3-24 03:04:08
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LIKEN
发表于 2025-3-24 08:08:54
Western Blotting: How It Began,the Nobel Prize for describing the molecular structure of DNA. The genetic code had been deciphered. Nobel laureate Dr. Frederick Sanger developed a method in the early 1950s to determine the amino acid units that made up a protein structure. Now, he was set to invent an elegant method to sequence DNA in 1977.
Musket
发表于 2025-3-24 13:16:10
Sample Preparation,source for Western blotting. One can purchase purified proteins commercially, or one can purify a specific protein in the laboratory. Cells or animal tissue can serve as a crude sample source for sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Several lysis buffers are available to extract and solubilize proteins from cells or tissues.
使高兴
发表于 2025-3-24 18:42:54
Things That Can Go Wrong in Western Blotting, Western blotting involves several steps beginning with making the gel. A mistake at any of these steps can affect the final result. This chapter details the problems arising during the Western blotting and subsequent immunostaining process and offers some ways to troubleshoot.
anniversary
发表于 2025-3-24 21:41:44
Concentrating Proteins for Protein Blotting,on and, with appropriate methods, helps remove unwanted salts or solvents. This chapter presents several available methods to concentrate proteins in the laboratory – all described recently by Dr. Dean Goldring.
脆弱么
发表于 2025-3-25 00:03:24
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